Application

Detect Histone-lysine N-methyltransferase NSD3 using this rabbit polyclonal antibody, Anti-Histone-lysine N-methyltransferase NSD3 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone-lys N-methyltransferase NSD3 in human and mouse cerebellum tissues.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone-lysine N-methyltransferase NSD3, also known as Nuclear SET domain-containing protein 3, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine or Wolf-Hirschhorn syndrome candidate 1-like protein 1, (WHSC1-like protein 1), and encoded by the gene WHSC1L1/NSD3, is a histone methylase that preferentially methylates Lys-4 and Lys-27 of histone H3. Lys-4 methylation is generally a specific tag for transcriptional activation while Lys-27 methylation is a mark for transcriptional repression. NSD3 is highly expressed in many tissues including the brain, heart and skeletal muscle. Overexpression of NSD3 is associated with certain forms of lung cancer as well as certain other cancers. NSD3 also facilitates the expression of many other genes important in cancer progression such as Brd4. Brd4 through its extraterminal domain interacts with NSD3 which regulates transcription by modifying the chromatin microenvironment at Brd4 target genes.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone-lys N-methyltransferase NSD3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in BALB/3T3 nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Histone-lys N-methyltransferase NSD3 in 10 µg of BALB/3T3 nuclear extract.

Specificity

This antibody is expected to cross react with isofomrs 1, 2, and 4 based upon immunogen design.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~180 kDa, ~160 kDa, ~90 kDa, and ~70 kDa observed. Uniprot desribes four isoforms at 162 kDa (isoform 1), 156 kDa (isoform 2), 73 kDa (isoform 3), and isoform 4 (155 kDa). This protein is known to be acetylated and phosphorylated and may be observed at higher molecular weights. Uncharacterized band(s) may be observed in some cell lysates.